HCV is a positive spherical enveloped single-stranded RNA viruses. HCV genome is a single strand RNA, total length of about 9500 bases. Genome sides were 5 'and 3' non-coding region, in the middle of an open reading frame (ORF), divided into structural and non-structural region area (Figure 1). Structural protein region including the core region (C) and two envelope protein region (E1, E2), respectively, encoding core protein and envelope proteins. Non-structural protein region including NS2, NS3, NS4 and NS5 region encoding functional proteins, such as proteases (NS2, NS3 and NS4A area), helicase (NS3) and RNA-dependent RNA polymerase (NS5B region). Although the non-structural protein is not part of the virus particle, but in viral replication plays a very important role. Now commonly used genetically engineered to express the structural and non-structural proteins as coating antigen to create anti-HCV ELISA detection method. HCV genome has significant heterogeneity, different sections of the same degree of variation in the genome there are significant differences. 5 'non-coding region most conservative, so PCR detection of HCV, RNA primer design more choice in the region.
Anti-HCV (Anti-HCV-Core) is the hepatitis C virus antibodies.
It is currently the most widely used for epidemiological investigation, clinical hepatitis C screening and diagnostic test items. So far the method detection of anti-HCV continuous improvement. With the improved method, the sensitivity and specificity of a gradual increase.
Referred to as the hepatitis C virus HCV, the classification of the virus are positive-strand RNA viruses, is considered to be caused by the early non-A, non-B hepatitis virus (non-A, non-B viral hepatitis) one of the major virulence factors. Was discovered in 1978, in 1989 obtained by gene technology was first genome sequence. It is a large 45 nm, with a housing, the single-stranded RNA virus, which belongs to the Flaviviridae virus is hepatitis. According to the 1994 establishment of the new classification system, HCV can be divided into six different genotypes (Genotype) and 30 subtypes (subtype). Mainly in Europe and Japan, mainly type 1, and the second type in Japan, China, mostly in the third type in Thailand, Singapore, India, and most of the more common in Egypt, the Middle East and central Africa, mainly type 4, The Type 5 and Type 6 is more common in South Africa and Southeast Asia. Hepatitis C only infect humans. As the name implies, namely anti-HCV hepatitis C virus antibodies.
No comments:
Post a Comment