What is Organic waste gas purification catalyst?

Organic waste gas purification catalyst carrier with ceramic honeycomb material, coating material unique to precious metal as the active component in the system, having a high catalytic activity, good thermal stability, long life, a small flow resistance , high strength and other characteristics. It is widely used in chemical, paint, wire, metal printing, color printing leather, pharmaceutical and urban sewage station sewer produce organic waste gas purification and deodorization treatment, in particular, can effectively purify acrylonitrile and acrylic acid plant emissions.

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From:Catalyst chemistry

Dengue Virus IgG ELISA Kit describes the relevant antibody

Dengue Virus belongs to the Flaviviridae family of a flavivirus serotype subgroup , morphology and Japanese encephalitis virus similar, but smaller, about 17 ~ 25nm, divided by 1,2,3,4 four different antigenic serotypes in different strains of the same type have different antigen . Among the most widespread type 2 , among various types of viral antigen with cross , and Japanese encephalitis virus and West Nile virus as well as some the same antigen . Virus in Aedes albopictus mosquitoes and passaged cells (C6/36 cells ) , monkey kidney, hamster kidney primary and passaged cells can proliferate and produce significant cytopathic .
Dengue virus is spread mainly through Aedes aegypti (Aedes ae-gyPti) and Aedes albopictus (Aedesalbopictus) and other media insects , causing dengue (dengue fever, DF) as well as the high morbidity and mortality rates of dengue hemorrhagic fever (dengue hemorrhagic fever, DHF) and dengue shock syndrome (dengue shock syndrome, DSS). Patients and latent infection is the major source of infection of the disease , while the jungle primate is to maintain the virus circulating in nature animal host . The heating of the patient's blood brain inoculated into naive mice can be isolated fixed .
These diseases are widely prevalent in tropical and subtropical regions , is a widely distributed , the incidence of many infectious diseases harmful to humans . In Asia , the Pacific Islands and Central and South America and many other countries have caused a serious threat. After World War II , the Japanese have had a pop. In recent years, Asia, Africa and tropical South America and sub-tropical regions the incidence of dengue virus infection in a clear upward trend . China in 1978 in Foshan, Guangdong first discovered the disease , later in the Hainan and Guangxi and other places have been found .


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From:Elisa assay kits

LPAP (Lymphocyte Phosphatase-Associated Phosphoprotein) Introduction

Human LPAP（Lymphocyte Phosphatase-Associated Phosphoprotein） is a phosphoprotein of alien action that noncovalently assembly with CD45 in lymphocytes. In CD45-deficient animal T cells, LPAP protein is actinic at accustomed levels but is added rapidly base than in wild-type cells. Announcement of CD45 cDNA rescues LPAP protein expression. This acerb suggests that LPAP is adequate from abasement through its alternation with CD45. We accept mapped the sites of alternation amid LPAP and CD45 employing chimeric CD45 molecules and LPAP abatement mutants. Our abstracts authenticate that the alternation amid LPAP and CD45 is advised via the transmembrane regions of both molecules. In addition, the intracytoplasmic amino acids adjoining to the transmembrane arena of LPAP may access its bounden to CD45.

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From:Biochemical Reagent

Insulin discovery process

Insulin is a pancreatic β cells by endogenous or exogenous substances such as glucose, lactose, ribose, arginine, such as glucagon stimulated secretion of a protein hormone. Insulin is the only way to reduce blood glucose in the body hormones, while promoting glycogen, fat, protein synthesis. Exogenous insulin is mainly used to treat diabetes, diabetic patients with early use of insulin and powerful antioxidants such as (alpha lipoic acid for injection, oral astaxanthin, etc.) is expected to appear longer honeymoon period, there will be no addiction and insulin injections dependence.
Discovery process

Insulin in 1921 by the Canadian FG Banting and CH Best first discovered. 1922 began to be used clinically, so that diabetic patients died in the past have been saved. Indications Kidney Institute, Chinese Academy of detection until the early 1980s, almost all insulin for clinical from pigs, cattle pancreas extract. Insulin composition of different animals in both differences pig and human insulin is most similar to the structure, only the B-chain of a different amino acids carboxyl terminal. In the early 1980s has been successfully used genetic engineering techniques of mass production by microorganisms human insulin, and has been used clinically.
1955 British F. Sanger team determined the complete amino acid sequence of bovine insulin, human knowledge has opened up the road of the chemical structure of protein molecules. September 17, 1965, Chinese scientists have synthesized a full biological activity of crystalline bovine insulin, it is the first one in the laboratory using artificial methods of protein synthesis and later the United States and the Federal German scientists also completed a similar work.
The early 1970s, the UK and Chinese scientists has successfully used X-ray diffraction method of three-dimensional structure of porcine insulin. These efforts for the further study of molecular structure and function of insulin relationship foundation. People use chemical synthesis and semi-synthetic analogs prepared to study the structural changes on the biological functions of; for different species of comparative study of insulin; study disease abnormal insulin molecule, i.e. the insulin gene mutations of individual amino acids in the insulin molecule arising from a change in molecular disease. These studies illustrate some of the causes of diabetes also has important practical significance.



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From: Biochemical Reagent 

Macrophage culture method

Macrophage is located within tissue leukocytes, monocytes derived cells, and monocytes from the bone marrow and the precursor cells. Macrophages and monocytes are phagocytic cells, non-specific defense in vertebrates (innate) and specific defense (immune). Their main function is to fix the cells in the form of free cells or cell debris and the phagocytosis of pathogens (i.e. phagocytosis and digestion), and activated lymphocytes or other immune cells, making it react to pathogens.
Culture methods
Macrophage cell line also has unlimited, mostly from the mouse, such as P338D1, S774A.1, RAW309Cr.l, etc., were vicious, macrophages were cultured in cell morphology and phagocytic function, easy passage and sidewall separation, but difficult to construct strains. Cultured macrophages in various ways and from various sources available to get the cells to mice derived method is most useful, and its method is as follows:
1, three days before the experiment, each mouse was intraperitoneally injected to thiolacetic acid sterile broth 1ml (Do not inject the intestines).
2, hoping to kill animals, portable mouse tail will all be immersed in 70% ethanol for 3 to 5 seconds.
3, set the animal on the autopsy table, with a needle fixed limbs, hands holding tweezers pull to the side torn skin, exposing the peritoneum, but not to hurt the peritoneal wall.
4, and then 70% alcohol scrub peritoneal wall, the use of a syringe was injected into the abdominal cavity suction 10mlEagle while rubbing pressure from both sides of the peritoneal wall with your fingers, so that adequate flow of fluid in the abdominal cavity.
5, gently stir the abdominal wall with a needle, the animal body slightly to one side, so that the fluid collection in the abdominal cavity under the needle into the needle tube drawing.
6, carefully pull out the needle, inject the liquid centrifuge tube, 250g4 ℃ for 10 min, the supernatant, add 10mlEagle medium.
7, cells were counted per mouse can produce 20 ~ 30 × 105 cells, of which 90% macrophages.
8, to obtain tie with 3 × 105 cells / square centimeter, to be inoculated with 2.5 × 106/ml. 9, the cell culture was purified to remove other white blood cells, a few hours after inoculation, culture solution, rinsed with Eagle 1 to 2 times, the addition of a new culture medium Eagle set 37 ℃ CO2 culture incubator.

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From:Biochemical Reagent

Lysozyme preparation

Lysozyme, also known as muramidase (muramidase) or N-acetyl murein polysaccharide hydrolase (N-acetylmuramide glycanohydrlase), is a kind of bacteria in the hydrolysis of the enzyme alkaline mucopolysaccharides. Primarily through the destruction of the cell wall of N-acetyl muramic acid and N-acetyl glucosamine between the β-1, 4 glycosidic bond, so that the insoluble cell wall mucopolysaccharides into soluble glycopeptides contents escape rupture cell walls leaving bacteria dissolved. Lysozyme can be negatively charged with direct binding of viral proteins, and DNA, RNA, apoprotein form complex salts, the virus inactivation. Thus, the enzyme has antibacterial, anti-inflammatory, anti-viral effects.
Lysozyme is a clone of biological engineering technology, extraction and preparation, it is a natural enzyme, safety green additive, no resistance.
The enzyme exists in a variety of human tissues, birds and poultry egg white, mammal tears, saliva, plasma, urine, milk and other body fluids and micro-organisms also contain this enzyme, of which the most abundant egg white. Extracted from the isolated egg lysozyme is 18 kinds of amino acid residues 129 a single polypeptide chain. It is rich in basic amino acids, 4 bonds maintain the enzyme conformation, is a basic protein, the N-terminal lysine, C terminal leucine. Decomposable Micrococcus lysodeikticus, Bacillus megaterium, yellow Sarcina other Gram-positive bacteria.
Lysozyme less than the isoelectric point wide pH range, with a positive charge molecules can be adsorbed on the weakly acidic cation exchange resin, eluted salting, lysozyme can be obtained precipitate was then purified product can be obtained. Adsorption resin egg wash ↓ 724 ↓ ↓ eluted with a buffer solution of ammonium sulfate salting-dialysis ↓ NaOH to basic protein precipitate freeze dried powder was dried lysozyme Lysozyme 5 ~ 10 ℃ under 540kg fresh egg white added to the processed Good 80kg 724 resin and stirred adsorbed 6h, allowed to stand at 0 ~ 5 ℃ overnight. Decanted upper egg white, centrifugal drying resin, washed repeatedly with distilled water adhesion egg protein, and the resin into the column with a 0? 15mol / L, pH = 6? 5 phosphate buffer solution, about 150L resin was washed, then Approximately 10% ammonium sulfate solution 600L eluate. Eluate was added ammonium sulfate to give a final volume of 40% with ammonium sulfate, a white precipitate formed, cool place overnight. Siphon supernatant drained precipitate filtered off with suction, dissolved in distilled water and then with 1 X precipitate was thin paste, and then into the dialysis bags, about 5 ℃ conditions, dialyzed against distilled water for about 24h, the intermediate changing the water 2-3 times . Precipitate was removed by centrifugation, and then a small amount of precipitate was washed once by centrifugation was combined with the washings. Beyond dialysis supernatant was slowly added 1mol / L sodium hydroxide solution, while constantly stirring, the pH increased to 8? 0 ~ 9? 0, if the white precipitate is removed by centrifugation. Then 3mol / L hydrochloric acid to adjust pH = 5? 0, freeze-drying, white sheet of lysozyme. Can also be used centrifugate 3mol / L hydrochloric acid pH = 3? 5, was added slowly with stirring a 5% solid sodium chloride, at a temperature of about 5 ℃ stand 48h, centrifuged, washed with acetone precipitate 0 ℃, drying, lysozyme.


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From: Biochemical Reagent

For Prostate Stem Cell Antigen-related information

Prostate stem cell antigen (PSCA) is a 1998 Reiter et al study gene expression in prostate cancer discovered during a tumor-associated antigen, belonging Ly-6/Thy-1 family, is a 123 amino acid glycoprotein. Because PSCA with stem cell antigen -2 (SCA-2) having a homology of 30%, which is called the prostate "stem cell antigen", but the current study, PSCA expressed mainly in cell differentiation and maturation of stem cells than the surface of the surface, their specific function is unclear. PSCA in prostate cancer, bladder cancer, and pancreatic cancer, increased expression and found its expression levels and prostate cancer clinical stage and metastasis, so PSCA has become of these tumor diagnosis and prognosis of biological markers. In addition to PSCA also become the target of these tumors immunotherapy Points, particularly in the treatment of prostate cancer, immune show great potential for clinical application. PSCA as a target in prostate cancer immunotherapy preclinical studies, the use of transgenic mice or mouse prostate cancer model for preclinical evaluation. To further explore the purpose of PSCA in prostate cancer immune RT-PCR amplification of mouse prostate stem cell antigen (mPSCA) gene prokaryotic expression vector pET-42a-mPSCA, induced mPSCA protein expression and purification, and to detect mPSCA antigenic activity. Methods RT-PCR method from the mouse prostate cancer cell line RM-1 cells was amplified mPSCA gene sequencing PCR method to remove mPSCA after the signal peptide sequence was cloned into the prokaryotic expression vector pET-42a to construct recombinant plasmid pET -42a-mPSCA. To convert To E. coli BL21 (DE3) induced expression, the fusion protein is then purified. Analyzed by SDS-PAGE, Western blot detection of purified protein, the purified protein further package board after its antigenic activity by ELISA were evaluated. Results sequencing results confirmed length mPSCA successfully amplified gene; digestion and sequencing Results confirmed pET-42a-mPSCA prokaryotic expression vector was successfully constructed; transformation can be successfully induced and purified proteins consistent with the expected size; Western blot test confirmed the purified protein could react with the specific antibody; ELISA assay showed purified mPSCA immunogenic antigens. Conclusion successfully amplified full-length mouse PSCA gene was successfully constructed mPSCA prokaryotic expression vector obtained purified mPSCA protein, which has a good antigenicity for further research to PSCA as a target for immunotherapy of prostate cancer foundation.


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From:Cytokines products