The adeptness of Clostridium sprogenes to acclimate to advance at low pH was advised in amazon serum able by enzymatic hydrolysis of canned tomatoes and amazon juice. The everyman pH at whicn C. sporogenes could abound afore adjustment in amazon serum, Trypticase Peptone Glucose Yeast extract (TPGY) and aqueous thioglycollate broths was pH 5.4.
Tomato serum and TPGY or aqueous thioglycollate borsch adapted to capricious pH levels were inoculated with C. sporogenes from tubes with advance at the lower pH values.
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