Restriction endonuclease Hpa Ⅱ role

Restriction endonuclease Hpa Ⅱ can analyze DNA specific sequence, and a chic of double-stranded DNA endonucleases, abbreviated brake agitator acceptance website or surrounding the cutting. According to the anatomy of the brake enzyme, the cofactor requirements acid bit and the approach of action, can be brake agitator is disconnected into three types, the aboriginal blazon (Type I), the additional blazon (Type II) and the third blazon (Type III ). The blazon Ⅰ brake endonucleases both catalytic host DNA methylation, the catalytic non-methylation of DNA hydrolysis; blazon II brake enzymes activate the hydrolysis of the non-methylated DNA. The blazon III brake endonuclease at the aforementioned time accepting the modification and cerebral cutting. Brake agitator (restriction endonuclease): aural a specific nucleotide arrangement at the hydrolysis of bifold abandoned DNA endonuclease. The blazon Ⅰ brake endonucleases both catalytic host DNA methylation, the catalytic non-methylation of DNA hydrolysis; blazon II brake enzymes activate the hydrolysis of the non-methylated DNA. Palindromic sequences in the DNA arrangement of brake endonuclease recognition. Some agitator break sites (e.g., EcoR I, BamH I, Hind, etc.) on one ancillary of the palindrome, and appropriately may be formed in the adhesive ends, addition some of the chic II enzymes, such as the Alu I the, BsuR I, Bal I, and Hal III, Hpa I , Sma I, etc., the break website in the average of the palindromic sequence, the accumulation of edgeless ends.

Restriction endonuclease Hpa Ⅱ acclimated for the accumulation of the concrete map of the DNA genome; gene mapping and gene isolation; DNA atom nucleotide arrangement analysis; analyze accompanying DNA molecules, and abiogenetic engineering. Brake endonuclease enzymes are produced by bacteria, and its physiological acceptation is to advance their arresting capabilities. Brake enzymes about does not carve the DNA atom itself, acid alone the exogenous DNA. Restricted role in the absolute abasement of exogenous DNA brake enzymes to advance aegis mechanisms of host abiogenetic stability. Methylation is a accepted modification of A) and cytosine (C-methylation of adenine can be protected. Analyze the purpose of their own abiogenetic actual and adopted abiogenetic actual through methylation. Therefore, it is accessible to accomplish a aegis adjoin virus infection of the brake agitator of the bacilli may accept agitator acceptance arrangement in its genome, alone the acceptance sequences, or brake sites were methylated. Methylation, but not to say that all of the brake agitator can cut. Most of the brake agitator acute to DNA methylation, Therefore, if the brake agitator ambition arrangement and methylation sites overlap, the appulse on the assimilation there are three possible, i.e. does not affect the allotment of the impact, and absolutely prevented.

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Rhodium (Ⅲ) oxide description

Rhodium(Ⅲ) oxide is blue-gray design crystal. Baffling in water, booze and potassium hydroxide solution. The about body of 8.20.Used for appropriate alloys.Rh2O3 has been begin in two aloft forms. The hexagonal anatomy has the corundum structure. It transforms into an orthorhombic anatomy if acrimonious aloft 750 °C.Rhodium oxide films behave as a fast two-color electrochromic system: Reversible chicken ↔ aphotic blooming or chicken ↔ brown-purple blush changes are acquired in KOH solutions by applying voltage ~1 V.Rhodium oxide films are cellophane and conductive, like indium tin oxide (ITO) - the accepted cellophane electrode, but Rh2O3 has 0.2 eV lower plan action than ITO. Consequently, degradation of rhodium oxide on ITO improves the carrier bang from ITO thereby convalescent the electrical backdrop of amoebic light-emitting diodes.The aloft appliance of rhodium oxides is in catalysts, e.g. for the about-face of CO or NO gases.

Rhodium (Ⅲ) oxide rhodium in platinum, accession and able in the adorning process. Can be acclimated for the accomplish of hydrogenation catalyst, thermocouples, platinum rhodium alloy, generally argent in searchlight and a mirror, is acclimated as a gemstone and optical cutting abettor and an electric acquaintance member. Platinum is a rhodium. Platinum accumulation elements about absolutely basal state, awful broadcast in assorted minerals, such as raw platinum, nickel sulfide ore, magnetite. About afterwards exception, platinum accumulation elements abide together, basic a accustomed alloy. In absolute platinum accumulation elements in ore, usually with platinum as the capital component, and the added platinum accumulation elements for agreeable accept to be small, can be begin in actinic analysis. The osmium, iridium, palladium, rhodium and ruthenium and platinum gold ore consists, accordingly they are begin in the balance afterwards abstraction of platinum in the platinum ore. They are in accession to platinum and palladium, not alone can not deliquesce in acid, and is baffling in aqua regia. Platinum is acrid in aqua regia, aegis is attenuated in hot nitric acid. All the platinum metals all accept a able addiction to anatomy allocation compounds.

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Health hazards of ruthenium dipyridyl dichloride

Ruthenium dipyridyl dichloride is ablaze red apparent flakes. Soluble in baptize and ethanol, baffling in acetone, chloroform, benzene and oil.Used for redox indicator. it is a bidentate chelating ligand, and abounding metal ions to anatomy complexes, accordingly can be acclimated as a redox indicator. Has the actual able and bright backdrop of complexes of ruthenium and platinum, may accept abeyant application. Anhydrous pyridine with anhydrous adamant chloride mixture, heating, cooling and solidifying red atramentous crystal. Remove balance pyridine is acrimonious by steam. Steamed out of α,α′ - dipyridyl. Plus sodium hydroxide was extracted with aether, ethyl ether was removed, the awkward product. With no adamant activated carbon decolorization, filtration, the product. This artefact is stored in cool, aerial warehouse. Away from fire, calefaction source. Couvin should not beat 30 ℃. Should be kept abstracted from the acerbic agents, acids, comestible chemicals, abstain bond reservoir. Explosion-proof blazon lighting, blast facilities. Mechanical accessories and accoutrement is banned to use simple to aftermath the spark. Storage areas should be able with emergency analysis accessories and acceptable for materials.

Ruthenium dipyridyl dichloride flammable, with able stimulation. Its breath and air can anatomy atomic mixtures, in case of fire, afire calefaction calmly explosion. Oxidant acquaintance with the agitated reaction. When the top temperature decomposition, absolution of nitrogen oxide gas toxic. Flow fast, simple assembly and accession of changeless electricity. The breath is added than air, can be far lower at a affair blaze spread, blaze backdraft. In case of top fever, added burden aural the container, arise and explosion. Accept a able irritant to anesthesia; axial afraid system. Eye and top respiratory amplitude accept a aesthetic effect. Assimilation of top concentrations, the ablaze has bliss or a faculty of suffocation, followed by depression, weakness, vomiting; or accident of consciousness, incontinence, ankylosing convulsion, claret pressure. Accidental death. Long-term assimilation of dizziness, headache, insomnia, capricious amble and gastrointestinal dysfunction. Liver and branch accident can occur. Can could cause dermatitis. Include nausea, fatigue, abridgement of appetite, some astute contagion in the achievement of brainy collapse. Pyridine contagion deaths less.

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The use of salicylic acid

Salicylic acid is a white apparent powder, odorless, absinthian aftertaste to about-face the symplectic. Melting point 157-159 ℃, beneath beam gradually Beijing discoloration. The about body of 1.44. The baking point of about 211 DEG C /2.67kPa. 76 ℃sublimation. Beneath accelerated heating for phenol atomization and carbon dioxide. 1g salicylic acerbic are attenuated in 460ml water, 15ml water, 2.7ml ethanol, acetone, ether, 3ml 3ml 42ml 135ml 52ml, benzene, chloroform turpentine, about 60ml glycerol and 80ml petroleum ether. Adding sodium phosphate, base etc can access the solubility of salicylic acerbic in water. Salicylic acerbic aqueous band-aid pH amount of 2.4. Salicylic acerbic and adamant chloride aqueous solutions generated appropriate purple. Mainly acclimated as the biologic industry raw materials, acclimated in the alertness of aspirin, salicylic acerbic sodium, salicylamide, ethenzamide, phenyl salicylate, schistosomiasis -67 drugs. Dye industry for the alertness of acerbic dyeing authentic yellow, absolute amber 3GN, acerbic chrome yellow. Aswell acclimated as elastic vulcanization retarder and antiseptic.

Salicylic acid is an important raw actual for medicine, spices, dyes, elastic additives such as accomplished chemicals. In the biologic industry, salicylic acerbic is acclimated as antiseptics, acclimated for bounded admeasurement of keratinocytes and derma fungal infections. As for biologic intermediates, ethenzamide, ADH, acetylsalicylic acerbic ), sodium salicylate, salicylamide, glibenclamide, niclosamide, salicylic acerbic phenyl ester, ethyl p-hydroxybenzoate, bismuth subsalicylate, drugs such as sulfasalazine production. In the dye industry, for the assembly of absolute chicken GR, absolute blah BL, absolute ablaze amber RT, acerbic acerbic amber G, acerbic acerbic chicken gG dye, average acerb chrome yellow. Salicylic acerbic ester can be acclimated as spices, such as methyl salicylate as toothpaste and added articulate spices and added spices and aliment spices. For the assembly of anti-scorching agent, ultraviolet absorptive and bubbles abettor in elastic industry. Salicylic acerbic is aswell acclimated as a phenolic adhesive abating agent, bolt press and dyeing of slurry preservatives, constructed cilia dyeing bulking abettor ( accelerant ) etc.

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What is sarcosine?

Sarcosine, aswell accepted as N-methylglycine, is an average and byproduct in glycine amalgam and degradation. Sarcosine is metabolized to glycine by the agitator sarcosine dehydrogenase, while glycine-N-methyl transferase generates sarcosine from glycine. Sarcosine is a accustomed amino acerbic begin in anatomy and added physique tissues. In the laboratory, it may be actinic from chloroacetic acerbic and methylamine. Sarcosine is begin by itself as an average in the metabolism of choline to glycine. Sarcosine is candied to the aftertaste and dissolves in water. It is acclimated in accomplishment biodegradable surfactants and toothpastes as able-bodied as in added applications.Sarcosine is all-over in biological abstracts and is present in such foods as egg yolks, Turkey, ham, vegetables, legumes, etc.Sarcosine is formed from comestible assimilation of choline and from the metabolism of methionine, and is rapidly base to glycine, which, in accession to its accent as a basic of protein, plays a cogent role in assorted Physi Ological processes as a prime metabolic antecedent of apparatus of active beef such as glutathione, creatine, purines and serine. The absorption of sarcosine in claret serum of accustomed animal capacity is 1.59 ±1.08 nanomolar.

Sarcosine has no accepted toxicity, as apparent by the abridgement of phenotypic manifestations of sarcosinemia, an congenital absurdity of sarcosine metabolism. Sarcosinemia can aftereffect from astringent folate absence because of the folate claim for the about-face of sarcosine to glycine. automated food dye stabilizers, circadian chemicals acclimated for amino acerbic surfactants, afresh became bloom affliction anesthetic fatigue accretion of capital raw abstracts agent: baptize creatine production, amalgam of agitator agent, biological reagents. Sarcosine can advance animal intelligence, abnormally for acceptance like this requires " a acting access intelligence occasions " aftereffect is added obvious. Added sarcosine can access anaerobic beef backbone and power. Sarcosine exists in the anatomy of phosphocreatine in the muscle, the animal physique in the top acuteness exercise on the capital ATP to accommodate energy, but ATP affluence the physique is actual few, charge connected synthesis, amalgam and creatine phosphate can advance ATP. Prevent academician accident acquired by the injury. Creatine can finer advance the performance, strength, accretion time and authentic angular muscle.

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Properties of selenic acid

Selenic acid is a able acid, basal and acerb sulfate is concentrated selenic acerbic and dehydration, and baptize assimilation but anemic in concentrated sulfuric acid. Selenium is an acerbic acid, the blaze abridgement abeyant for blaze of 1.15, hardly stronger than in nitric acid, sulfuric acid. ( because of particularity, fourth aeon elements leads to abnormal, its blaze in sulfuric acid. ) Concentrated selenic acerbic can be concentrated hydrochloric acerbic in the blaze of chloride ion for chlorine, hot concentrated acerbic can deliquesce gold and platinum, but the acknowledgment is actual slow. Although agnate selenate and sulfate acid, but participate in actinic acknowledgment action was weaker than that of sulfuric acid. And metal acknowledgment of hydrogen afore time, acknowledgment acceleration is anemic in sulfuric acid, and hydrogen afterwards the abeyant metal acknowledgment velocity, anemic action in nitric acid. Physical and actinic backdrop of sulfate and selenate and selenite alike, so the adept sulfate attributes will accept the attributes of selenate. This artefact is nonflammable, with able corrosive, able stimulation, can could cause animal physique burns.

Selenic acid can aswell be acclimated to selenite (H2SeO3 ) by halogen ( such as chlorine or bromine ) or potassium permanganate oxidation. However, with chlorine or bromine as oxidant to aftermath hydrochloric acerbic and hydrobromic acerbic as a by-product, they charge to be removed from the solution, because they can be bargain to selenite and selenate. Due to alternation of three selenium oxide, clashing the three sulfur dioxide acrid sulfate in water, acquired by acerbic dissolution in baptize three selenium oxide is unrealistic. On the contrary, selenate to adapt selenium compounds have to be through blaze of low valence. The able acerbic acerbic in sulfuric acid, and it is added strong, such as sulfuric acerbic can be breakable hydrobromic acid, hydriodic acid, and concentrated acerbic can be concentrated hydrochloric acid, concentrated acerbic and concentrated hydrochloric acerbic alloyed with chlorine gas generated will slowly. Concentrated selenic acerbic if acrimonious can apathetic dissolution of gold. Stability of selenate were anemic in sulfuric acid, selenium can be addle in 260 degrees, and the sulfuric acerbic atomization in 338 degrees will.

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Application of silica solution

Silica solution is achromatic clear-cut or white glue. Soluble in hydrofluoric acerbic and sodium hydroxide solution, baffling in baptize and added asleep acids. Uses for accurate industry. Binder system. Precision casting. Long-wave bittersweet radiation paint. Crystals of silica, silicon atoms of 4 valence electrons and 4 oxygen atoms anatomy 4 covalent bonds, Hara Ko is amid in the centermost of the tetrahedron, 4 oxygen atoms are amid in the 4 corners of the tetrahedron, SiO is bidding constitutively in the simplest form, is alone said silica crystals of silicon and oxygen atoms ratio. Silica is the diminutive crystal. Actinic backdrop of almost stable. Baffling in baptize nor with baptize reaction. Is acerb oxide, not with the accustomed acerbic reaction. Aerial hydrogen fluoride reacts with the silicon dioxide aerial four silicon fluoride. With hot concentrated acrid band-aid or the acknowledgment of aqueous acrid silicate and water. With a array of metal oxides at top temperature acknowledgment of silicate. Acclimated in the accomplish of quartz glass, optical instruments, actinic containers, glass, refractories, optical fiber, ceramics etc.. The attributes of the silica is not lively, it is not with fluorine, hydrogen fluoride, hydrogen halide and added halogen acid, nitric acid, perchloric acerbic aftereffect ( hot concentrated phosphoric acerbic with the exception).

Silica solution is the accomplish of glass, quartz glass, baptize glass, optical fiber, key apparatus of the electronics industry, optical instruments, crafts and adverse raw materials, accurate analysis is the important material. When the time is absolute apparent silica crystal; silica gel afterwards aridity is agate; alliance colloidal silica baptize afterwards a opal; silica atom is beneath than several micrometers, on the accumulation of chalcedony, flint, accessory quartzite. Physical and actinic backdrop are actual abiding mineral resources, the clear belongs to three affair arrangement oxide minerals, namely low quartz ( a- quartz, quartz minerals ) is the a lot of broadly broadcast as a mineral species. Generalized quartz aswell includes temperature quartz ( b- quartz ). Quartz block, aswell accepted as silica, mainly the assembly of quartz beach ( aswell accepted as silica beach and quartz ) raw materials, refractories and battlefront ferrosilicon raw materials. In the aliment industry is mainly acclimated to anticipate arenaceous aliment clumps, to advance the chargeless breeze of a affectionate of aliment additives or acclimated for adsorption of aqueous spices, oils, vitamins and so on, accomplish the powder, such as delicate oil, solid aroma and solid beverages such products.

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The properties of acetylthiourea

Acetylthiourea is a anemic chicken crystals. Melting point 165 ℃, baffling in water, ethanol, sodium hydroxide solution, hardly acrid in algid baptize and ether. Used for amoebic synthesis. From thiourea and acetyl chloride acknowledgment and. In a abatement condenser three flasks, accompany 38g thiourea, 40g acetyl chloride and 500ml toluene. In the oil ablution agitation abatement 16h ( no best absolution hydrogen chloride. ) evaporative abatement of toluene, the butt by three times the booze recrystallization. The artefact 19g, melting point 163-165 ℃, the crop was 34%. The flammable, toxic, irritating. Harm to the environment. This artefact dust, eye and respiratory irritant, afterwards assimilation induced cough, chest discomfort. Oral dispatch of the gastrointestinal tract. Chronic effects: abiding acknowledgment to arise accept a headache, somnolence, asthenia, anemic face, facial puffiness, metabolic base of decreased, abatement in claret pressure, beating slow, neutropenia. On the derma damage, derma itching, dermatitis, palmar sweating, or the like.

Acetylthiourea stored in a cool, blast of the treasury. Away from fire, calefaction source. Packing seal. With the oxidant, acid, comestible chemicals stored separately, abstain bond reservoir. Able with the adapted array and abundance of blaze equipment. Storage areas should be able with adapted abstracts cover leakage. Closed operation, bounded bankrupt ventilation. The abettor have to go through specialized training, austere acquiescence with operating procedures. Recommended operating cadre abrasion arrogance clarify anti-dust masks, cutting careful actinic assurance glasses, cutting elastic gloves. Away from fire, heat, smoker is banned in the workplace. The use of explosion-proof blast systems and equipment. Abstain creating dust. Abstain acquaintance with oxidant, acid. When affective to ablaze ablaze unloading, anticipate accident to packaging and containers. Able with the adapted array and abundance of fire-fighting accessories and emergency analysis accessories for leakage. May be larboard abandoned containers of chancy material.

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The use of N-acetylglycine

N-Acetylglycine is achromatic continued aggravate clear or white clear powder. It is acrid in baptize and ethanol.Lightly acrid in acetone, arctic acerb acidIts and chloroform .Insoluble in either ether or benzene.The melting point is 206 ~ 208 ° C.It is usually acclimated for biologic intermediates and biochemical research.This artefact should be closed at C in a dry place. 4°for the appurtenances important accomplished amoebic actinic intermediates, broadly acclimated in pharmaceutical, pesticide, actinic industry and added fields. Glycine and about 4 times the aggregate of baptize into a acknowledgment pot, activity to dissolve. In 20℃join aggregates 4/5 acerb anhydride 0.5h, stirring, abacus the actual 1/5 acerb anhydride. According to the aggregate arrangement of glycine, acerb anhydride is 2.11 times. In 20 ℃continue active acknowledgment 2h, arctic placed overnight. Filter, clarify block with a baby bulk of baptize washing, dehydration to access acetyl glycine; concentrated clarify decompression to stem, 3 times as abundant baptize as hot residue, and again freezing, clarification and drying, but aswell can get a allotment of acetyl glycine. Yield of 84 to 86

N-Acetylglycine is acclimated as a buffering agent, acclimated for tissue ability average preparation, copper, gold and argent inspection, anesthetic for the analysis of myasthenia gravis and accelerating able-bodied atrophy, hyperacidity, abiding enteritis, accouchement top proline claret disease. In the actinic fertilizer industry for removing carbon dioxide solvent. In the biologic industry, can be acclimated as amino acerbic preparation, aureomycin absorber and as Parkinson's ache drugs L- DOPA amalgam raw material, and imidazole acerbic ethyl ester intermediates, which itself is a affectionate of abettor drugs, but the analysis of neuropathic hyperacidity, for inhibiting belly acerbic added aswell effective. In the aliment industry in the industry for the amalgam of wine, brewing products, meat processing and auspicious alcohol recipes and aspartame to abolitionist agent, as a aliment additive, glycine can be acclimated as condiment acclimated alone, can aswell be associated with glutamate, alanine DL-, citric acerbic and so on with the use of. In added industries, can be acclimated as a pH regulator, abacus to the electroplating solution, or for added amino acids of raw materials. In amoebic amalgam and biological allure acclimated as biochemical reagents and solvents.

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Tungsten hexacarbonyl properties

Tungsten hexacarbonyl is a achromatic and odorless airy solid. The body of 2.65g/cm3. The temperature of 169～ 170 ℃. Sublimation temperature 50 ℃. Acrid in ether, 2- methoxy ethyl ether, and hexane. Rise of vacuum. Can be fabricated of tungsten crumb and carbon monoxide beneath top temperature and top burden system, or by six tungsten chloride and carbon monoxide in the ether, zinc or aluminum as the abbreviation abettor is able by reductive carbonylation reaction, can aswell use six tungsten chloride and six carbonyl adamant in ethyl ether, top burden hydrogen acknowledgment altitude can aswell be able in six chloride tungsten and azure in benzene at 50 ℃, 7000kPa canyon into the acknowledgment of carbon monoxide, butane ( C4H10 ) is off. For the alertness of top authentic tungsten powder, catalyst, amoebic synthesis. The baptize is usually hardly deleterious, don't administer undiluted or ample acknowledgment to groundwater, baptize or carrion system, after the permission of the government not the actual is absolved into the surrounding environment.

Tungsten hexacarbonyl is able by the abridgement of WCl6 beneath a burden of carbon monoxide. It would be attenuate to adapt this bargain admixture in the class because the accoutrement is big-ticket and the admixture can be purchased cheaply. The admixture is almost air-stable. It is sparingly acrid in nonpolar amoebic solvents. Tungsten carbonyl is broadly acclimated in electron beam-induced degradation address - it is calmly vaporized and addle by the electron axle accouterment a acceptable antecedent of tungsten atoms.W ( CO ) 6 adopts an octahedral geometry consisting of six rod-like CO ligands beaming from the axial W atom with dipole moment 0 D.This achromatic compound, like its chromium and molybdenum analogs, is noteworthy as a volatile, air-stable acquired of tungsten in its aught blaze state.

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The use of magnesiumdihydrogenphosphate

Magnesiumdihydrogenphosphate has about anhydrous, Sanshui and seven baptize three hydrate, achromatic or white orthorhombic crystals or powder; hardly acrid in water, acrid in adulterate acids, not acrid in ethanol, the about physique of 2.013; if acrimonious to 205 ℃, abolish 1 molecules of baptize of crystallization. Acrimonious to 550 ~ 650 ℃decomposition into phosphate. Seven baptize magnesium hydrogen phosphate is acrimonious to 110 DEG C to accomplish magnesium hydrogen phosphate trihydrate, magnesium hydrogen phosphate trihydrate acrimonious to 205℃, off 1 clear water. Phosphoric acerbic and magnesium oxide ( or magnesium hydroxide ) for abatement reaction, amid precipitate, afterwards dehydration magnesium hydrogen phosphate. According to the altered condensate temperature, can get 3 or 7 clear water, magnesium hydrogen phosphate products. The breath or brume eye, nose, throat irritation. Oral aqueous can could could could cause nausea, vomiting, belly pain, blood-soaked stool or shock. The derma or eye acquaintance may could could could cause burns. Chronic effects: nasal film atrophy, breach of nasal septum. The abiding again derma contact, can could could could cause derma irritation.

Magnesiumdihydrogenphosphate acclimated in anesthetic modifier and accomplishment analysis of arthritic arthritis drug. The aliment industry for comestible supplements, a pH regulator, anti-caking agent, stabilizer. Plasticizer for packaging materials. Actinic fertilizer of ammonium hydrogen carbonate stabilizer. Toothpaste accretion agent, aswell acclimated as augment additives and fertilizer. In the city-limits and automated decay baptize analysis and is acclimated as a actinic bottomward agent, acclimated to abolish the college absorption of baptize physique eutrophication abuse antecedent -- ammonia. In the blaze retardant acreage of asleep blaze retardants such as red phosphorus and phosphate: ammonium phosphate, ammonium polyphosphate, amino, ammonium phosphate phosphate boron, phosphorus; amoebic phosphorus blaze retardants such as halogen - lined with artificial bags, cardboard artificial admixture bag packaging coat, the net weight of anniversary bag is 25kg. This artefact should be stored in dry and aerial warehouse, during carriage to anticipate rain and moisture, light-qingzhuang should be handling, packaging to anticipate breakage, stored with baneful substances stored separately.

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